FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3286.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3286.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1531423
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1469259.594018112565895No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG368602.4069117415632393No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG102590.6698998251952595No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG49180.3211392280251766No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG45030.29404024884045754No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG41180.26890023200644103No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGTTTTTTTTTGGGGGGGGGG18700.12210865319379426No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTTA109850.068.7884553
GGGTTAA111300.068.64699654
GGTTAAA112550.068.25999555
GTTAAAA118650.066.34357556
GGGGGTT123050.066.04771452
TTTGGGG210000.064.42856638
TTGGGGG211850.064.3057739
TTTTGGG215650.062.25354837
TGGGGGG228950.060.86153440
GGGGGGT144750.060.15819551
AGGGGGG262400.059.41054570
GGGGCCG21900.059.29154645
GGCCGGG21350.058.8517747
TTTTTGG231000.058.04477736
GGGCCGG22450.057.83707446
CCGGGGA14350.057.8023149
GCCGGGG21900.057.21581348
AAGGGGG288200.053.7727769
AAAGGGG288250.053.5154768
GGTTTAA11250.053.2011255