FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3285.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3285.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2197667
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1161775.286378691585212No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG292091.3290912590488004No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC72060.32789317034837395No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG66400.3021385860551212No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG64820.29494914379658066No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG50070.22783251511716746No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG26090.11871680286412818No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA94600.068.5155154
GGGGTTA94600.068.1424553
GGTTAAA95900.067.96406655
GGGGGTT105150.065.0286652
TTGGGGG175300.064.1686339
TTTGGGG175400.063.76455338
GTTAAAA105450.063.70105456
TGGGGGG192450.060.67040340
TTTTGGG187550.058.47649837
GGGGGGT126050.058.21637351
GGGGCCG21450.058.0939745
AGGGGGG223450.057.3466370
GGGCCGG21900.057.2199246
GCCGGGG21300.056.86232848
GGCCGGG21900.056.2622147
CCGGGGA15300.053.98209449
AAGGGGG242250.052.65177569
AAAGGGG242000.052.56632268
TTTTTGG213900.051.26469436
AAAAGGG249250.050.88050567