FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3283.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3283.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1489862
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG973706.53550463063022No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG246101.6518308407087365No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG65860.4420543647666697No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG35600.2389483052792809No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG33020.2216312651775802No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG28410.19068880205012276No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTTA73150.068.3076853
GGGTTAA73800.068.2750154
GGTTAAA74900.067.7086855
GGGGGTT81650.065.5678352
TTTGGGG143050.063.99037638
TTGGGGG144050.063.89991839
GTTAAAA81850.063.6698556
TGGGGGG156700.060.32520740
TTTTGGG150550.059.70771837
GGGGGGT95750.059.67846351
GGGCCGG17200.059.42320346
AGGGGGG174700.059.31023470
GGCCGGG17200.057.593647
GGGGCCG18100.057.4334545
GCCGGGG17400.056.1250348
TTTTTGG167000.053.7616436
AAGGGGG192650.053.61691769
AAAGGGG192850.053.45240868
AAAAGGG198650.051.96223467
CCGGGGA12400.051.92902449