FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3280.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3280.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919396
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1755779.14751307182051No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG425132.2149155255090665No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG110400.5751809423381106No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC104220.5429833135007054No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG79180.41252560701387314No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG67200.35011013881450204No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG41800.21777684229830635No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG21920.11420259289901616No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA138450.068.9395154
GGGGTTA137950.068.6547853
GGTTAAA140300.068.3779455
GGGGGTT150200.066.7354252
GTTAAAA148900.066.3073656
TTTGGGG260950.065.4962938
TTGGGGG263700.065.33092539
TTTTGGG266400.063.06421337
TGGGGGG287300.062.05983440
GGGGGGT177050.060.7660851
AGGGGGG326550.059.30750370
GGGGCCG29300.059.00823645
GGCCGGG28650.059.0001647
GCCGGGG28500.058.70129848
TTTTTGG286500.058.55887636
GGGCCGG29550.058.26605246
CCGGGGA18700.056.70930549
AAGGGGG353500.054.46634369
AAAGGGG353550.054.28469568
AAAAGGG357400.053.6803467