FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3279.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3279.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1511823
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1055986.984812375522796No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG256901.6992729969050608No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG70200.46434007155599566No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG52670.3483873442856737No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG42580.2816467271631666No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG24600.16271746097261386No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTTA80850.068.61465553
GGGTTAA81950.068.5432954
GGTTAAA83000.068.1422655
GGGGGTT90600.065.4757352
GTTAAAA88950.065.1951756
TTGGGGG158350.064.43426539
TTTGGGG157800.064.30820538
TGGGGGG171400.061.1212640
GGGGGGT107200.059.91330351
TTTTGGG166550.059.7086737
GGCCGGG16700.057.64040447
AGGGGGG201900.057.35276870
GGGGCCG17850.055.4938245
GCCGGGG17050.055.43251448
GGGCCGG17850.055.29772646
TTTTTGG184300.054.064936
AAGGGGG215650.053.41819469
AAAGGGG215400.053.3229968
AAAAGGG220900.051.91782467
GGTTTAA7550.051.455255