FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3278.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3278.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1580374
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG808225.114105901514451No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG210991.335063725421957No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG55680.35232166563104683No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG37290.2359568051613099No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG31880.20172440194536229No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG18800.11895918307944828No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA61450.068.4046854
GGTTAAA62250.067.7028455
GGGGTTA61350.067.5463553
GGGGGTT68500.064.0173452
GTTAAAA68050.063.2718556
TTTGGGG118900.063.20969438
TTGGGGG120250.062.9518639
TGGGGGG132950.059.12328740
TTTTGGG128550.057.5660537
GGGGGGT83400.057.11224751
AGGGGGG153900.057.0911370
AAGGGGG166250.052.57301369
GGCCGGG12650.052.5704347
GGGGCCG13450.052.5663245
AAAGGGG167900.051.85775868
GGGCCGG13700.050.83902746
TTTTTGG146300.050.40471336
GCCGGGG13150.050.3053948
AAAAGGG173000.050.20917567
CCGGGGA8800.049.70764549