FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3277.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3277.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2187597
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1064624.866618485945995No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG263901.2063465071491686No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC63760.29146136148477075No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG62650.28638730076883445No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG59610.27249077412338746No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG45110.20620799900530123No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG23170.10591530341283152No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA86050.068.2092754
GGGGTTA85350.068.07001553
GGTTAAA88250.066.98324655
GGGGGTT94550.065.36880552
TTGGGGG168050.063.84001539
TTTGGGG168050.063.3653138
GTTAAAA97000.062.9975756
TGGGGGG184350.060.05471440
TTTTGGG177800.058.43097337
GGGGCCG19050.058.42294345
GGGGGGT115000.058.06865351
AGGGGGG210100.057.32311670
GGGCCGG19250.057.26920746
GGCCGGG19850.054.30520647
AAGGGGG225150.053.14939569
AAAGGGG226650.052.63380468
GCCGGGG20300.052.58658648
TTTTTGG203100.051.02345336
AAAAGGG233800.051.0103767
CCGGGGA15500.048.77454849