FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3276.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3276.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1844353
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1165326.3183132513136036No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG293801.5929705430576468No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG75330.40843591221420195No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG49650.26920009347451385No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG42530.23059576989871247No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG30170.16358039919689996No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA90700.068.60869654
GGTTAAA91650.068.3194655
GGGGTTA90250.068.1327153
GGGGGTT98650.065.5240852
GTTAAAA98350.065.264956
TTGGGGG170100.063.9092439
TTTGGGG170400.063.40991638
TGGGGGG184550.060.34657740
TTTTGGG179650.059.3640537
GGGGGGT117200.059.30544351
AGGGGGG211200.058.63939370
GGGGCCG18750.057.48844545
GGGCCGG18800.056.77554346
GCCGGGG19250.054.3649248
GGCCGGG19350.054.07955647
AAGGGGG229650.053.69676269
AAAGGGG230150.053.44613668
TTTTTGG201350.052.94310436
AAAAGGG236450.052.0101367
CCGGGGA13600.050.95293449