FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3275.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3275.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1730654
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG997665.764641574803513No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG252111.45673254157099No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG67910.39239501367691054No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG37070.2141964829480647No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG35230.2035646639940739No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG27240.15739714581886385No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA79000.068.270454
GGTTAAA80250.067.78377555
GGGGTTA78550.067.6367153
GGGGGTT87100.064.89500452
TTTGGGG148900.063.88000538
TTGGGGG150350.063.50536339
GTTAAAA87800.063.43010356
TGGGGGG162300.060.53141440
GGGGCCG16450.060.005445
GGGGGGT102800.059.1699251
TTTTGGG158750.059.14459637
AGGGGGG186450.058.16361270
GGGCCGG17100.057.1087646
GGCCGGG16800.056.8784847
GCCGGGG17050.055.84081348
AAGGGGG204350.052.87881569
AAAGGGG205600.052.44270368
TTTTTGG182500.051.47414436
CCGGGGA12250.051.14006849
AAAAGGG211250.051.0747267