FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3271.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3271.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1984832
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1330776.7046984329152295No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG340541.7157119595008545No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG87520.44094412020765483No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG43670.22001862122335794No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG42090.21205824976622706No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG39010.19654056363460481No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA101900.068.451854
GGGGTTA101500.068.1698653
GGTTAAA103700.067.7750155
GGGGGTT112650.065.3305152
GTTAAAA110700.064.9105156
TTGGGGG190800.064.476139
TTTGGGG192350.063.6718338
TGGGGGG208950.060.22921440
TTTTGGG201750.059.8015437
AGGGGGG233350.059.0995370
GGGGGGT133150.058.9283551
GGGCCGG22600.057.6084646
GGGGCCG22800.057.10744545
GGCCGGG23100.055.60537747
GCCGGGG23250.054.3434248
CCGGGGA15550.054.0172749
TTTTTGG223950.053.9033636
AAGGGGG256250.053.528569
AAAGGGG257100.053.2466568
AAAAGGG263700.051.90069267