FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3269.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3269.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1945596
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1180216.066058935154061No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG288931.4850462274799086No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG81080.41673605414484816No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC69040.3548527032333537No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG54360.2794002454774784No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG45040.23149718646625506No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG29190.1500311472679837No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA91850.068.47227554
GGGGTTA91150.068.1551553
GGTTAAA93650.067.572555
GGGGGTT100000.065.4449752
TTGGGGG179050.064.5724439
TTTGGGG179600.063.81770338
GTTAAAA102250.063.70337756
TGGGGGG196400.060.82858740
TTTTGGG189500.059.44608737
GGGGGGT120050.058.27390351
AGGGGGG229350.057.71381870
GGGGCCG19600.056.9657745
GGGCCGG19550.056.03726646
GGCCGGG19250.055.9971447
GCCGGGG19100.055.3360648
TTTTTGG210650.053.5523136
AAGGGGG246850.053.41830469
AAAGGGG247150.053.24292468
AAAAGGG251600.052.28866267
CCGGGGA13250.051.50547449