FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3266.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3266.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1491423
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG946956.349305327864731No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG234631.573195532052275No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG61670.41349771325774104No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC56600.37950333339367837No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG45150.30273101594919755No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG36280.24325761370181365No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG22500.15086263253282267No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA71650.069.0723754
GGGGTTA71650.068.3396453
GGTTAAA73700.067.59212555
GGGGGTT80400.065.20972452
GTTAAAA79700.064.6537356
TTTGGGG138250.064.3317338
TTGGGGG139700.064.10853639
TGGGGGG153450.060.43951840
TTTTGGG146750.059.60579737
GGGGGGT94800.059.39845351
GGGCCGG15650.057.6962246
AGGGGGG176550.057.64159870
GGCCGGG15550.056.4917947
GCCGGGG15600.055.86203448
GGGGCCG16200.055.74114245
CCGGGGA11200.054.0556349
TTTTTGG163200.053.6781236
AAGGGGG191100.052.95799369
AAAGGGG191900.052.5236168
AAAAGGG196000.051.29469767