FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3265.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3265.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1752216
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1108916.328614736995895No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG281571.6069365877266273No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG69420.3961840321056308No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG46130.26326662922835997No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG42290.24135152287160944No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG28130.16053956818109183No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA85800.069.10380654
GGGGTTA86150.068.2542853
GGTTAAA87650.067.7611855
GGGGGTT95250.065.66334552
GTTAAAA95450.063.69053356
TTGGGGG169350.063.20703539
TTTGGGG168400.063.1930738
TTTTGGG175050.059.95252237
TGGGGGG184100.059.7936640
GGGGGGT113800.058.9888351
AGGGGGG209950.057.79137870
GGGCCGG17550.057.03479846
GGGGCCG18150.055.73102645
TTTTTGG189950.055.0144436
GGCCGGG17850.054.90137547
AAGGGGG224300.053.7663269
AAAGGGG223900.053.6652668
GCCGGGG18350.053.03154448
AAAAGGG229500.052.247567
AAAAAGG239400.050.23456666