FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3261.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3261.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2154133
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG21890610.16213947792453No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG529442.457786961157923No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG153540.7127693601091483No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC133050.6176498851277985No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG88400.41037391841636517No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG75740.35160317399157803No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG57240.2657217544134926No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG25240.11717010973788526No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA168400.069.17007454
GGGGTTA167650.068.8724753
GGTTAAA170650.068.5436255
GGGGGTT183650.066.68062652
GTTAAAA179850.066.6152156
TTTGGGG316450.065.2610738
TTGGGGG320400.064.7390539
TTTTGGG327750.062.1857937
TGGGGGG350900.060.62817440
GGGGGGT218050.059.91958251
GGCCGGG33350.059.7149847
AGGGGGG401350.059.0463370
GGGCCGG34800.058.83331346
TTTTTGG346100.058.83133736
GGGGCCG35300.058.1018345
CCGGGGA22950.057.9573149
GCCGGGG34050.057.67040648
GGTTTAA13100.055.30519555
GGGTTTA13250.054.68037454
AAGGGGG434550.054.2667869