FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3256.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3256.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2128321
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1702177.997712751037085No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG416601.9574114994871543No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG111790.5252497156209049No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG64880.30484123400558466No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG58880.27664999781517924No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG46510.21852906586929322No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA125600.069.13418654
GGGGTTA125300.068.5192353
GGTTAAA127450.068.3794355
GTTAAAA133750.066.5716556
GGGGGTT137900.066.29410652
TTGGGGG249100.064.72581539
TTTGGGG249450.064.3042638
TTTTGGG258350.061.31519737
TGGGGGG274100.060.36475840
GGGGCCG30100.058.9448945
GGGCCGG30050.058.5771446
GGCCGGG29550.058.50383847
GCCGGGG29400.058.4548648
GGGGGGT168050.058.35186451
AGGGGGG309900.058.16467770
CCGGGGA20300.057.06601349
TTTTTGG280000.056.4234136
AAGGGGG332150.053.86404469
AAAGGGG332400.053.73634368
AAAAGGG338150.052.843367
Produced by FastQC (version 0.11.5)