FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3253.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3253.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2412639
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG2087758.65338743177077No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG482211.998682770194795No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG139850.5796557213905603No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC120690.5002406078986537No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG111700.4629785061088708No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG84720.3511507523504345No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG46880.1943100480428278No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG26880.11141326986755996No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTATGGGGGGGGGG24920.10328938560638372No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA167450.068.8510154
GGGGTTA165900.068.7755653
GGTTAAA169050.068.58485455
GGGGGTT178700.067.1746552
GTTAAAA180350.065.89593556
TTGGGGG309750.065.68011539
TTTGGGG308900.065.418538
TTTTGGG315450.062.7840637
GGGGCCG31150.061.79445345
TGGGGGG344150.061.47554840
GCCGGGG30750.060.5546448
GGGGGGT214950.060.0955751
CCGGGGA21200.060.08864249
GGGCCGG32000.059.3874546
GGCCGGG31650.059.27013847
AGGGGGG399000.058.16050770
TTTTTGG342700.057.7605336
TATGGGG12250.056.86355238
GGGCCCC7000.054.9867949
AAGGGGG428800.053.85508369