FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3252.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3252.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2077026
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1662888.006062514383547No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG415572.0007934421620144No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG106500.5127523680493167No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC103500.49830863937187114No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG66210.31877309191122305No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG58780.28300079055341626No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG44640.2149226827203896No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTTA128100.068.7477253
GGGTTAA129250.068.6793154
GGTTAAA131100.068.1057255
GGGGGTT142350.065.8657552
GTTAAAA139700.065.8438656
TTTGGGG244550.064.3030738
TTGGGGG246150.064.18066439
TTTTGGG255300.060.71785737
TGGGGGG268350.060.5930540
GGGGGGT167450.059.35920351
AGGGGGG302200.058.9880570
GGCCGGG27250.058.94807447
GGGGCCG28400.058.90524745
GGGCCGG27950.058.84905246
GCCGGGG27350.057.97598348
TTTTTGG273850.056.52058836
CCGGGGA18100.055.1101549
AAGGGGG326950.054.10769769
AAAGGGG328550.053.82131668
AAAAGGG335550.052.5394767