FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3251.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3251.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2484836
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1555466.259809500506271No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG382971.5412284754406327No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG109350.44006928425055014No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG56670.22806334100117673No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG52230.2101949585405234No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG42190.1697898774808478No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA118500.068.6074354
GGGGTTA118700.067.9933453
GGTTAAA119800.067.9839155
GGGGGTT130800.064.9920252
GTTAAAA128800.064.9454356
TTGGGGG223650.064.4611739
TTTGGGG224650.063.93010338
TGGGGGG246400.060.16903340
TTTTGGG239150.059.39408537
AGGGGGG278100.058.87667570
GGGCCGG24750.058.82689346
GGGGGGT154900.058.54519751
GGGGCCG25350.057.2987945
GGCCGGG25450.057.07364747
GCCGGGG26150.054.87884548
AAGGGGG301950.053.9215669
AAAGGGG303000.053.59210668
TTTTTGG264250.053.5605736
CCGGGGA17750.052.6498349
AAAAGGG311150.052.2006667