FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3250.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3250.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1114377
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG17253415.482552134511032No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG398383.574912260393027No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC105440.9461788963698999No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG104900.9413331395030586No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG72530.6508569362074056No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG63660.5712608928576236No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG42190.37859718928154473No Hit
GGGGGGGGGGGCGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG20840.187010320564764No Hit
GGGGGGGGGGGGGGGGGGGGGGGGAAAAAAATTTTTTTTTGGGGGGGGGG12650.11351634141767103No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTATGGGGGGGGGG12480.11199082536699878No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGTTTTTTTTTGGGGGGGGGG11860.10642717859395878No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG11430.1025685203481407No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA132600.069.59852654
GGTTAAA134150.069.2900555
GGGGTTA132050.069.1993453
GGGGGTT140350.068.345652
GTTAAAA140350.068.0026356
TTTGGGG245400.067.1144638
TTGGGGG248900.066.5474539
TTTTGGG244950.066.09450537
CCGGGGA17100.064.062149
TTTTTGG253950.063.44838336
TGGGGGG270150.062.8935840
GGCCGGG26300.062.01863547
GGGGGGT166650.061.69648751
GGGCCGG26850.061.39451646
GGGGCCG27250.061.00705745
GCCGGGG26450.060.34359748
GGGCCCC5550.059.897079
GGCCCCC5650.059.46162410
TTTTTTG271050.059.1846635
AGGGGGG311750.059.15537670