FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n02_QG3249.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n02_QG3249.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2246322
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGGG1143655.091211322330459No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG289551.2889959676306426No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGGGGGGGGGGGGGGGGGGGG78050.3474568650442813No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCC69170.3079255779002298No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGGGGGGGGCG43350.1929821281187648No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGG39630.17642172404490541No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTGGGGGGGGGGG31150.13867112551094635No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAA92300.068.0281954
GGGGTTA91650.067.7468853
GGTTAAA93700.067.388355
GGGGGTT100600.065.12633552
TTGGGGG178300.063.3736139
GTTAAAA102550.063.0034356
TTTGGGG178850.062.88795538
TGGGGGG191700.060.34842340
GGGGGGT118950.058.3748951
AGGGGGG222300.057.8019170
TTTTGGG193000.057.56987437
GGGGCCG19050.056.2204945
GGGCCGG19200.055.59649746
GGCCGGG19600.053.7464247
AAGGGGG239050.053.4272769
GCCGGGG19650.053.25701548
AAAGGGG241500.052.70036768
AAAAGGG248100.051.2572467
TTTTTGG218650.050.790136
AAAAAGG261800.048.62843366