FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20504293
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG375720.18323967571083774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCAC697950.063.02128627
CCAGTCA731600.062.79807726
CTCCAGT789950.062.1459124
AGTCACC196700.061.50435628
GAACTCC844450.061.35989821
AGTCACT150250.060.93041228
AACTCCA860750.060.77999522
TGAACTC869400.060.68926620
CTGAACT876800.060.30684719
CGTCTGA890050.059.62383316
ACGTCTG891750.059.5850115
CACGTCT895550.058.97011614
GTCACCG51100.058.9690129
ACACGTC905400.058.57590513
CACACGT907000.058.4802912
GCACACG910000.058.2722611
GTCACGG62000.057.7464829
AGCACAC929650.057.49207710
TCCAGTC830700.057.22824525
GTCACCC39250.056.88825629
Produced by FastQC (version 0.11.5)