FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3511.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3511.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3359116
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCTCGTAT35571610.58957178019455TruSeq Adapter, Index 9 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG380750.068.0720148
GTATGCC381450.067.9787247
CGTATGC381650.067.877946
TCGTATG382500.067.7087645
ATGCCGT383800.067.5624249
TGCCGTC385950.067.1044450
GCCGTCT386350.067.0087751
CTCGTAT385050.066.7968144
CTGCTTG387750.066.7387659
TGCTTGA390150.066.3133260
CCGTCTT390750.066.2631952
TCTCGTA388800.066.1605743
ATCTCGT389200.066.065642
AATCTCG388100.065.9923241
TAGCCAA386150.065.9779936
ATAGCCA387300.065.9105635
GCCAATC385450.065.8548438
CAATCTC389450.065.681740
CCAATCT388100.065.631639
GCTTGAA394800.065.5677461
Produced by FastQC (version 0.11.5)