FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3508.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3508.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291311
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGTACAATCTCGTAT26741791.79776939422129TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGTACAATATCGTAT8630.2962469663006203TruSeq Adapter, Index 9 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8020.27530714597114425No Hit
GATTCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGTACAATCTCGTA5590.19189114039634617TruSeq Adapter, Index 9 (97% over 34bp)
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACGGTGTACAATCTCGTA4910.16854838986512696TruSeq Adapter, Index 9 (96% over 32bp)
GGTCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGTACAATCTCGTAT4820.1654589081771715TruSeq Adapter, Index 11 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGTACAATCTCGTATG4680.16065304777368516TruSeq Adapter, Index 9 (97% over 35bp)
GATCCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGTACAATCTCGTA4620.15859339331504818TruSeq Adapter, Index 11 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGAACATCTCGTATG3450.11843013137162689TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGGGTACAATCTCGTAT3300.11328099522503442TruSeq Adapter, Index 20 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGTACATCTCGTATG3290.11293771948192824TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGGA207.908123E-570.018891
GATCGGG353.6925485E-970.018891
GATTGGA150.002220854470.018891
GATTCGG550.070.018891
GGTCGGA252.831126E-670.018891
ACAGTGT150.002222367670.0068732
TTATGCT150.002222367670.0068757
CTTCAAA150.002222367670.0068762
TAAACTA150.002222367670.0068730
CCAATCA252.8340164E-670.0068726
ACGCTGT207.914852E-570.0068732
GTCACGT252.8340164E-670.0068729
GTCACGC353.6980055E-970.0068729
GTCACGA401.3460522E-1070.0068729
GTCACCG550.070.0068729
CAGTCAA401.3460522E-1070.0068727
GTCACAG150.002222367670.0068729
ACGTTGT150.002222367670.0068732
CACGTTG252.8340164E-670.0068731
CCAGTAC600.070.0068726
Produced by FastQC (version 0.11.5)