FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3506.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3506.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2186419
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACATCAATCTCGTAT35710416.33282550142493TruSeq Adapter, Index 4 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC378100.068.5702446
GTATGCC378150.068.5534947
TATGCCG378500.068.5009148
ATGCCGT379550.068.32062549
TCGTATG379950.068.2379245
CTCGTAT380000.067.9894944
GCCGTCT381800.067.86159551
CTGCTTG381900.067.828659
TGCCGTC382500.067.7526150
TGCTTGA383250.067.6291560
CCGTCTT383450.067.56045552
TCTCGTA382450.067.51887543
ATCTCGT382450.067.4807342
TCTGCTT385150.067.2879858
AATCTCG384700.066.9768841
GCTTGAA387100.066.9474961
CAATCTC385250.066.6601840
TCAATCT385700.066.33893639
CTTCTGC391700.066.1911156
TTCTGCT392050.066.1766657
Produced by FastQC (version 0.11.5)