FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3505.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3505.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2317946
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTGAAGATCTCGTAT28782512.41724354234309TruSeq Adapter, Index 10 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC306400.068.3262546
GTATGCC306500.068.3168447
TCGTATG307100.068.17895545
TATGCCG307100.068.1749148
CTCGTAT308150.067.84589444
ATGCCGT308900.067.7849649
CTGCTTG310150.067.54999559
GCCGTCT310500.067.4429451
TGCCGTC310750.067.4406550
TCTCGTA310550.067.2652243
TGCTTGA311700.067.2575660
CCGTCTT312550.067.08044452
GATCTCG310700.066.9863941
ATCTCGT312200.066.8775342
AGATCTC313450.066.35403440
GCTTGAA316250.066.3327561
TCTGCTT315900.066.32188458
AAGATCT315150.065.8406439
CTGGTGA315050.065.7187833
ACTGGTG315900.065.6416832
Produced by FastQC (version 0.11.5)