FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3500.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3500.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3394535
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAATGTCATCTCGTAT42761712.597218764867648TruSeq Adapter, Index 2 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC465600.068.2413147
TATGCCG465600.068.2247948
CGTATGC465600.068.198746
ATGCCGT467700.067.9064949
TCGTATG467100.067.9047745
TGCCGTC470250.067.51691450
CTCGTAT469000.067.5028244
CTGCTTG470350.067.41672559
GCCGTCT470900.067.3939951
TGCTTGA473150.067.08581560
TCTCGTA473350.066.82332643
CCGTCTT475450.066.794252
GCTTGAA477650.066.5133961
TCTGCTT477350.066.4647758
ATCTCGT476250.066.44581642
CATCTCG477350.065.9114641
GTCATCT478150.065.1799939
CTTCTGC488500.064.984556
TTCTGCT489850.064.734957
TGTCATC481100.064.67216538
Produced by FastQC (version 0.11.5)