FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3498.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3498.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3270964
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGCAATATCTCGTAT34182310.450222014060687TruSeq Adapter, Index 7 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG365050.067.5495648
CGTATGC365350.067.5347646
GTATGCC365700.067.4540847
TCGTATG367000.067.19298645
ATGCCGT367450.067.0923849
TGCCGTC369300.066.7119550
CTCGTAT369250.066.706744
GCCGTCT369650.066.6876851
CTGCTTG371200.066.40922559
TGCTTGA371900.066.3211560
TCTCGTA373650.065.9305543
CCGTCTT374250.065.8867252
TATCTCG373350.065.7679341
ATCTCGT376400.065.4385642
GCTTGAA378150.065.2342661
TCTGCTT378850.065.09595558
CGCAATA376250.064.73575636
GCAATAT378200.064.54610437
ATATCTC380750.064.3938540
AATATCT380650.064.35560639
Produced by FastQC (version 0.11.5)