FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3497.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3497.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2798000
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCATTAATCTCGTAT42148615.063831308077196TruSeq Adapter, Index 7 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC455450.068.15587647
CGTATGC455400.068.1479946
TATGCCG455950.068.0912548
TCGTATG457750.067.7880645
ATGCCGT458000.067.78367649
CTCGTAT457950.067.4527644
TGCCGTC460050.067.452450
CTGCTTG460450.067.30897559
GCCGTCT461700.067.2125551
TGCTTGA462350.067.0718160
CCGTCTT462850.067.04674552
TCTCGTA460600.066.98868643
TTAATCT457550.066.8068139
TAATCTC459950.066.70170640
ATCTCGT462600.066.692742
TCTGCTT465600.066.589458
ATTAATC459900.066.4666338
AATCTCG463600.066.435641
GCTTGAA467050.066.4343261
CATTAAT463600.066.0906837
Produced by FastQC (version 0.11.5)