FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3494.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3494.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180162
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCGGTTATCTCGTAT16804693.27494144159145TruSeq Adapter, Index 27 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCAGTTATCTCGTAT6240.3463549472141739TruSeq Adapter, Index 27 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4900.2719774425239507No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCGATTATCTCGTAT4080.22646285010157527TruSeq Adapter, Index 27 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCGGGTATCTCGTAT3780.20981116994704765TruSeq Adapter, Index 27 (97% over 37bp)
GATTCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCGGTTATCTCGTA3230.17928308966374706TruSeq Adapter, Index 27 (97% over 35bp)
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACCTTCGGTTATCTCGTA3060.16984713757618145TruSeq Adapter, Index 27 (96% over 33bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCGGTTATCTCGTATG3060.16984713757618145TruSeq Adapter, Index 27 (97% over 36bp)
GATCCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCGGTTATCTCGTA2460.13654377726712627TruSeq Adapter, Index 12 (97% over 34bp)
GGTCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCGGTTATCTCGTAT2230.12377748914865509TruSeq Adapter, Index 12 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCGGTATCTCGTATG2150.11933704110744775TruSeq Adapter, Index 27 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCAGC150.002219125270.0216656
CATGAAA150.002219125270.0216662
CACATTC150.002219125270.0216631
TTCCGCT150.002219125270.0216657
ACCATCG301.01719706E-770.0216632
CACCGTC150.002219125270.0216631
TTCTGCA207.898388E-570.0216657
ACCGTCG150.002219125270.0216632
TCAACAA150.002219125270.0216630
TCAGCTT150.002219125270.0216658
TGCCCTC207.898388E-570.0216650
ACCTGCG353.6816346E-970.0216632
CCTCTTC301.01719706E-770.0216653
TAACCTT150.002219125270.0216630
CACCTTG207.898388E-570.0216631
CACCTTA207.898388E-570.0216631
TCTGCAT207.898388E-570.0216658
CACCTGC401.3460522E-1070.0216631
CACCTAC150.002219125270.0216631
ATGCCCT207.898388E-570.0216649
Produced by FastQC (version 0.11.5)