FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3490.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3490.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1124364
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTTGAACATCTCGTAT38425534.17532044782651TruSeq Adapter, Index 11 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12530.11144077896481923No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG402050.068.7204448
GTATGCC402200.068.68564647
ATGCCGT402450.068.6295649
CGTATGC402700.068.620846
TGCCGTC402650.068.60721650
GCCGTCT403150.068.5195551
TCGTATG403450.068.4758845
CTGCTTG402750.068.4480759
TGCTTGA403100.068.4372460
CCGTCTT404600.068.3085952
CTCGTAT404250.068.2970844
GCTTGAA404550.068.20363661
TCTCGTA405500.068.05202543
TCTGCTT405450.068.03239458
CTTCTGC407250.067.80649656
ATCTCGT407250.067.7510142
TTCTGCT407450.067.6928657
CGTCTTC408050.067.685653
CATCTCG407050.067.5835441
GTCTTCT409300.067.4758954
Produced by FastQC (version 0.11.5)