FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3489.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3489.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2390833
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGCGAATCTCGTAT30991112.962469566046645TruSeq Adapter, Index 2 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC335000.068.1058846
TATGCCG335250.068.0579448
GTATGCC335300.068.0553847
ATGCCGT336300.067.9045949
TCGTATG335800.067.8929445
CTCGTAT335600.067.66083544
CTGCTTG338050.067.3798559
TGCCGTC339050.067.3359950
TGCTTGA339250.067.1888960
GCCGTCT340000.067.1272651
TCTCGTA339000.066.97330543
CCGTCTT341500.066.8221552
GCGAATC337550.066.4923338
ATCTCGT342150.066.37717442
GCTTGAA344400.066.2945961
TCTGCTT344000.066.27547558
AATCTCG341700.066.17779541
CGAATCT341450.065.8442439
TGCGAAT342500.065.7152937
CTTCTGC348700.065.36072556
Produced by FastQC (version 0.11.5)