FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3488.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3488.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3248877
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCATCGTATCTCGTAT3026399.315187986495026TruSeq Adapter, Index 1 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC328850.068.0541447
TATGCCG328950.068.0067748
CGTATGC329100.067.96844546
ATGCCGT331150.067.5379949
TCGTATG331550.067.4757145
TGCCGTC332450.067.2169250
CTGCTTG333300.066.9458159
CTCGTAT334400.066.8692344
GCCGTCT334950.066.66501651
CGTATCT332450.066.5237839
TGCTTGA336500.066.32590560
TCTCGTA337350.066.2222443
GTATCTC335350.066.10503440
ATCTCGT338750.065.9238342
CCGTCTT338800.065.9188152
TATCTCG337350.065.8322941
TCGTATC337300.065.3286338
ATCGTAT337400.065.30492437
GCTTGAA342950.065.1213161
TCTGCTT343500.065.0108658
Produced by FastQC (version 0.11.5)