FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3485.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3485.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2037959
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTTGAATCTCGTAT27145613.319993189264355TruSeq Adapter, Index 5 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG288350.067.8161148
GTATGCC288600.067.76948547
CGTATGC289000.067.6498146
TCGTATG289450.067.4946145
ATGCCGT289500.067.47748649
TGCCGTC290850.067.153950
CTCGTAT289850.067.0392244
CTGCTTG291150.066.9180359
GCCGTCT292900.066.6838951
TGCTTGA293000.066.5791460
TCTCGTA292050.066.4726743
CCGTCTT294550.066.26443552
TCTGCTT294200.066.2599958
GCTTGAA295350.066.1086661
AATCTCG292500.066.0593141
ATCTCGT293950.066.0072942
GAATCTC297000.064.8313440
CTTCTGC300950.064.7955156
TTCTGCT302250.064.518457
TTGAATC298050.064.3931738
Produced by FastQC (version 0.11.5)