FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3481.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3481.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2926098
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACGGTTATCTCGTAT2337207.987428992467101TruSeq Adapter, Index 9 (97% over 37bp)
CTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCT51670.17658328600067394No Hit
CCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCC38870.13283902316327068No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG35410.1210144021150351No Hit
CTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCT34860.11913476582124044No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC34320.11728930473278748No Hit
GCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGC33960.11605899734048553No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC252700.067.17126546
TATGCCG253500.066.9776848
GTATGCC253300.066.97185547
ATGCCGT254850.066.5736349
TCGTATG255550.066.4073145
CTCGTAT257750.065.7341244
CTGCTTG259250.065.4178559
TGCCGTC259950.065.2686250
GCCGTCT260250.065.1821751
TGCTTGA260700.065.0775260
TCTCGTA261950.064.6946343
ACGGTTA258450.064.6034536
CGGTTAT258600.064.57178537
GGTTATC259300.064.51784538
CCGTCTT263700.064.35593452
ATCTCGT263200.064.335342
GTTATCT260750.064.2943939
TATCTCG262800.064.2201241
TCTGCTT265550.063.90431258
TTATCTC263500.063.7960640