Basic Statistics
Measure | Value |
---|---|
Filename | HMT3KBGX7_n01_QG3481.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 2926098 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACGGTTATCTCGTAT | 233720 | 7.987428992467101 | TruSeq Adapter, Index 9 (97% over 37bp) |
CTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCT | 5167 | 0.17658328600067394 | No Hit |
CCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCC | 3887 | 0.13283902316327068 | No Hit |
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG | 3541 | 0.1210144021150351 | No Hit |
CTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCT | 3486 | 0.11913476582124044 | No Hit |
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC | 3432 | 0.11728930473278748 | No Hit |
GCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGC | 3396 | 0.11605899734048553 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTATGC | 25270 | 0.0 | 67.171265 | 46 |
TATGCCG | 25350 | 0.0 | 66.97768 | 48 |
GTATGCC | 25330 | 0.0 | 66.971855 | 47 |
ATGCCGT | 25485 | 0.0 | 66.57363 | 49 |
TCGTATG | 25555 | 0.0 | 66.40731 | 45 |
CTCGTAT | 25775 | 0.0 | 65.73412 | 44 |
CTGCTTG | 25925 | 0.0 | 65.41785 | 59 |
TGCCGTC | 25995 | 0.0 | 65.26862 | 50 |
GCCGTCT | 26025 | 0.0 | 65.18217 | 51 |
TGCTTGA | 26070 | 0.0 | 65.07752 | 60 |
TCTCGTA | 26195 | 0.0 | 64.69463 | 43 |
ACGGTTA | 25845 | 0.0 | 64.60345 | 36 |
CGGTTAT | 25860 | 0.0 | 64.571785 | 37 |
GGTTATC | 25930 | 0.0 | 64.517845 | 38 |
CCGTCTT | 26370 | 0.0 | 64.355934 | 52 |
ATCTCGT | 26320 | 0.0 | 64.3353 | 42 |
GTTATCT | 26075 | 0.0 | 64.29439 | 39 |
TATCTCG | 26280 | 0.0 | 64.22012 | 41 |
TCTGCTT | 26555 | 0.0 | 63.904312 | 58 |
TTATCTC | 26350 | 0.0 | 63.79606 | 40 |