FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3480.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3480.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2835142
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAAGGTTATCTCGTAT49174017.344457526289688TruSeq Adapter, Index 2 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG528600.068.4871548
GTATGCC529150.068.4135547
CGTATGC530050.068.30698446
TCGTATG530050.068.30698445
ATGCCGT531000.068.2141849
CTGCTTG533300.067.91222459
TGCCGTC533800.067.8402850
CTCGTAT532750.067.8007344
GCCGTCT534350.067.7716551
TGCTTGA535450.067.6579560
CCGTCTT537450.067.41212552
TCTCGTA535800.067.3286743
ATCTCGT536250.067.2917542
GTTATCT532250.067.2403439
TCTGCTT539050.067.2232258
TATCTCG535100.067.2062541
GGTTATC532400.067.0941438
GCTTGAA540650.067.0654961
AGGTTAT533600.067.0320637
TTATCTC535450.066.91456640
Produced by FastQC (version 0.11.5)