FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3479.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3479.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2971542
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACACCTATCTCGTAT35342211.89355560177174TruSeq Adapter, Index 18 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC378400.068.40031447
TATGCCG378650.068.37015548
CGTATGC379200.068.2629546
ATGCCGT380300.068.0723749
TCGTATG381500.067.8686145
TGCCGTC382600.067.65742550
GCCGTCT383200.067.58230651
CTCGTAT382350.067.50719544
CTGCTTG383550.067.415759
TGCTTGA384850.067.2289360
CCGTCTT388500.066.6693452
TCTCGTA386700.066.6199743
TCTGCTT389150.066.4613258
CCTATCT383300.066.4314639
GCTTGAA389950.066.4046461
ATCTCGT388800.066.27814542
TATCTCG387850.066.0964841
CTATCTC386500.066.08858540
CTTCTGC397500.065.1279556
ACCTATC391000.064.9911538
Produced by FastQC (version 0.11.5)