FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3477.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3477.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2653839
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCGACTTATCTCGTAT1490795.617484708002256TruSeq Adapter, Index 12 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC160950.067.0189446
TATGCCG160900.067.0013148
GTATGCC161250.066.89677447
TCGTATG163150.066.1758345
ATGCCGT163800.065.9080149
CTCGTAT163750.065.61153444
CTGCTTG165200.065.1437159
TGCCGTC165800.065.1365550
GCCGTCT165900.065.0413451
TGCTTGA166750.064.6346260
CGACTTA164900.064.2122536
TCTCGTA168250.063.75509643
GACTTAT166400.063.7079537
CCGTCTT169900.063.52946552
TATCTCG168900.063.363541
TCTGCTT170350.063.27108858
ATCTCGT170400.062.98937642
GCTTGAA172200.062.60813561
TTATCTC171450.062.0909840
CTTATCT171650.061.8159239
Produced by FastQC (version 0.11.5)