FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3476.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3476.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2020458
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCACTGATCTCGTAT29696414.697855634712525TruSeq Adapter, Index 27 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC313100.068.5137247
CGTATGC313100.068.5008446
TATGCCG313300.068.46049548
TCGTATG314050.068.3159245
ATGCCGT315300.068.04240449
TGCCGTC316400.067.7998150
CTGCTTG316050.067.7940159
CTCGTAT316250.067.6874144
GCCGTCT317450.067.57555451
TGCTTGA317850.067.4524760
TCTCGTA318700.067.09019543
CCGTCTT320000.066.9823752
ATCTCGT319050.066.939842
GATCTCG318650.066.8480841
TCTGCTT320900.066.7333558
GCTTGAA321950.066.658761
CTGATCT319000.066.26675439
TGATCTC320050.066.1805840
ACTGATC319150.066.1462538
CTTCTGC324350.066.1115156
Produced by FastQC (version 0.11.5)