FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3474.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3474.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3398951
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCTTGCATCTCGTAT38548011.341146135969597TruSeq Adapter, Index 9 (97% over 37bp)
CTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCT41570.12230243978215631No Hit
CCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCC35970.10582676831763682No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC407200.068.4138547
CGTATGC407350.068.3770546
TATGCCG407800.068.29146648
ATGCCGT408100.068.2215649
TCGTATG409400.068.0024745
TGCCGTC410750.067.77888550
CTCGTAT410400.067.7173944
GCCGTCT413850.067.2582251
CTGCTTG413850.067.2562359
TCTCGTA413900.067.1193943
TGCTTGA415750.066.9692160
CCGTCTT417800.066.6716352
ATCTCGT417900.066.4853242
GCATCTC415100.066.4681340
TGCATCT416350.066.159339
CTTGCAT415550.066.13055437
TCTGCTT421200.066.08066658
GCTTGAA423150.065.86520461
CATCTCG420550.065.84904541
TTGCATC417950.065.8055538
Produced by FastQC (version 0.11.5)