FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3473.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3473.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2128948
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCCAATCTCGTAT31637314.860532056208042TruSeq Adapter, Index 23 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC334150.068.1653247
TATGCCG334300.068.1588848
CGTATGC334700.068.0412546
ATGCCGT335650.067.879149
TCGTATG335350.067.8587845
TGCCGTC336850.067.61967550
CTGCTTG337500.067.4567459
GCCGTCT337600.067.4311551
CTCGTAT336000.067.38376644
TGCTTGA338950.067.2047460
CCGTCTT339200.067.123452
TCTCGTA338750.066.78194443
GCTTGAA342200.066.5971661
TCTGCTT342700.066.4944758
ATCTCGT340250.066.4772442
AATCTCG340250.066.2216641
CCAATCT338800.066.09186639
CTTCTGC345150.065.9662656
CAATCTC340950.065.95225540
TTCTGCT345700.065.8932457
Produced by FastQC (version 0.11.5)