FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3472.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3472.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2449365
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACACCATCTCGTAT2276049.292367613646803TruSeq Adapter, Index 7 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC241850.068.0731347
TATGCCG242550.067.8650148
CGTATGC242900.067.8076846
ATGCCGT243900.067.4805449
TCGTATG244350.067.42100545
TGCCGTC245550.067.0440850
CTCGTAT245750.066.8958744
GCCGTCT246050.066.8536751
CTGCTTG247700.066.5637959
TGCTTGA248900.066.2488260
CCGTCTT248600.066.2383352
TCTCGTA249200.065.8854743
TCTGCTT252750.065.21998658
ATCTCGT251600.065.2000142
GCTTGAA253300.065.1131861
ACCATCT251400.064.6592439
CATCTCG253100.064.57851441
ACACCAT252150.064.4834337
CTTCTGC257150.064.1163356
CACCATC253250.064.0612338
Produced by FastQC (version 0.11.5)