FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3471.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3471.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2523595
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTAAGCATCTCGTAT31821312.609511431113155TruSeq Adapter, Index 11 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC335850.068.49290547
TATGCCG336100.068.44330648
CGTATGC336350.068.41054546
ATGCCGT337350.068.18835449
TCGTATG338850.067.914845
TGCCGTC339500.067.7489150
CTCGTAT339050.067.6036544
GCCGTCT340600.067.593151
CTGCTTG341800.067.3544659
TGCTTGA342200.067.29085560
TCTCGTA341750.067.0401643
CCGTCTT344450.066.799652
GCTTGAA345350.066.7061661
GCATCTC342450.066.5338740
ATCTCGT344750.066.50622642
TAAGCAT343300.066.3385537
TCTGCTT347600.066.2205358
CATCTCG345950.066.1035441
AGCATCT344850.065.9997739
CTAAGCA345800.065.8945336
Produced by FastQC (version 0.11.5)