FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3469.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3469.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1671952
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACGTGCATCTCGTAT33311519.9237179057772TruSeq Adapter, Index 3 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC352800.068.3982246
TATGCCG352650.068.373948
GTATGCC352700.068.3700447
TCGTATG353400.068.2701545
ATGCCGT353350.068.24457649
CTCGTAT353800.068.0643744
TGCCGTC354800.067.9775750
GCCGTCT355000.067.9433451
CTGCTTG355150.067.8986959
TGCTTGA356300.067.70698560
CCGTCTT357000.067.5725152
TCTGCTT357450.067.51076558
TCTCGTA356850.067.4237843
GTGCATC354100.067.3287938
GCTTGAA358350.067.3253961
ATCTCGT357500.067.29140542
GCATCTC355900.067.2714340
CGTGCAT354600.067.2338537
TGCATCT355500.067.1797739
CATCTCG358200.066.9938141
Produced by FastQC (version 0.11.5)