FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3467.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3467.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2360170
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATCTCATCTCGTAT25838310.947643601943929TruSeq Adapter, Index 22 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC279500.068.0608947
CGTATGC279900.067.9982546
TATGCCG279700.067.9871948
ATGCCGT281250.067.6225449
TCGTATG281450.067.62234545
TGCCGTC282900.067.2309950
CTCGTAT282650.067.1880944
GCCGTCT283650.067.06840551
CTGCTTG283950.067.0231659
TGCTTGA285500.066.6399860
CCGTCTT285950.066.52894652
TCTCGTA285600.066.3932443
ATCTCGT287450.065.9659442
GCTTGAA289200.065.8357961
TCTGCTT289350.065.8100358
CATCTCG288700.065.5726141
CTTCTGC294200.064.7146156
TTCTGCT296700.064.20470457
TCATCTC293600.064.0583140
ATCTCAT292950.063.867337
Produced by FastQC (version 0.11.5)