FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3465.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3465.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3105788
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCATACATCTCGTAT34018310.953194487196164TruSeq Adapter, Index 10 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG366100.067.92004448
CGTATGC366500.067.830946
GTATGCC367250.067.7400347
ATGCCGT367400.067.61520449
TCGTATG368050.067.53355445
CTGCTTG369800.067.1195959
TGCCGTC370050.067.0796450
CTCGTAT370300.066.9908944
GCCGTCT371500.066.81782551
TGCTTGA372200.066.701360
CCGTCTT374300.066.3377652
TCTCGTA374100.066.2251543
TCTGCTT377200.065.84099658
GCTTGAA377900.065.7230161
ATCTCGT378350.065.453542
CTTCTGC383850.064.7501156
CATCTCG382350.064.5124641
ACATCTC382550.064.3496240
TACATCT381900.064.3114839
TTCTGCT387400.064.162757
Produced by FastQC (version 0.11.5)