FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3462.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3462.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2698863
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCTCGTAT43538216.132052645873465TruSeq Adapter, Index 11 (97% over 39bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC471800.068.2962547
TATGCCG472150.068.25429548
CGTATGC472400.068.2205246
TCGTATG474150.067.96747645
ATGCCGT474400.067.9208549
CTCGTAT473100.067.7854344
TGCCGTC475550.067.72356450
GCCGTCT476850.067.5745651
CTGCTTG477050.067.5632459
TGCTTGA477400.067.5147860
TCTCGTA476250.067.3015943
CCGTCTT479800.067.1895252
GCTTGAA480950.067.0091761
ATCTCGT478600.067.0064542
AATCTCG477550.066.9106941
TCTGCTT482100.066.8760658
CAATCTC478250.066.6480540
CCAATCT478300.066.46548539
ACCAATC480150.066.16688538
TACCAAT482400.066.06988537
Produced by FastQC (version 0.11.5)