FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3460.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3460.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2361839
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCAAGTTATCTCGTAT28379412.015806327188264TruSeq Adapter, Index 6 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG306900.067.9102548
CGTATGC307750.067.7225846
GTATGCC307850.067.672247
ATGCCGT308150.067.62627449
TCGTATG309800.067.2377145
CTGCTTG309850.067.2072159
CTCGTAT309850.067.10261544
TGCCGTC310650.067.09614650
GCCGTCT311150.066.9658351
TGCTTGA311450.066.8899960
CCGTCTT314100.066.3605952
TCTCGTA313750.066.17927643
TCTGCTT315450.066.0709958
ATCTCGT314150.066.0615942
TATCTCG313750.065.9115741
GCTTGAA316200.065.8962361
GTTATCT312850.065.5754239
TTATCTC314950.065.34653540
CTTCTGC321300.064.858556
TTCTGCT323050.064.550557
Produced by FastQC (version 0.11.5)