FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3459.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3459.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2016727
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCGAACAATCTCGTAT22522811.167996461593463TruSeq Adapter, Index 12 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC240650.068.0122547
TATGCCG240900.067.95956448
CGTATGC240850.067.9557746
TCGTATG241500.067.7661445
ATGCCGT242250.067.6258749
CTCGTAT242350.067.2651944
TGCCGTC243750.067.1970250
CTGCTTG244650.066.83369459
TGCTTGA245100.066.7027160
GCCGTCT245850.066.6405851
TCTCGTA245600.066.3465743
AATCTCG245200.066.2549841
CCGTCTT247350.066.2489652
ATCTCGT246450.066.0609442
GCTTGAA248800.065.7248261
CAATCTC247500.065.5386740
TCTGCTT249700.065.4944358
ACAATCT247900.065.152239
AACAATC250700.064.3423638
CTTCTGC254900.064.28351656
Produced by FastQC (version 0.11.5)