FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3457.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3457.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1612258
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATAGCCATCTCGTAT31014119.236437344395252TruSeq Adapter, Index 2 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG329950.068.3588748
GTATGCC330200.068.34527647
CGTATGC330350.068.3142446
ATGCCGT330150.068.3132249
TCGTATG331300.068.0739845
TGCCGTC331350.068.07004550
CTGCTTG331750.067.9162259
CTCGTAT331650.067.9050644
GCCGTCT332600.067.7931651
TGCTTGA332900.067.72156560
TCTCGTA333250.067.49711643
CCGTCTT334000.067.4670852
TCTGCTT335200.067.2380958
ATCTCGT334450.067.2130842
GCTTGAA336000.067.15925661
CATCTCG334050.067.0127641
CTTCTGC338350.066.5934956
CCATCTC335150.066.5548240
GCCATCT334200.066.47175639
TTCTGCT340350.066.2124557
Produced by FastQC (version 0.11.5)