FastQCFastQC Report
Tue 17 Dec 2019
HMT3KBGX7_n01_QG3455.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMT3KBGX7_n01_QG3455.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1704451
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGAAGCAATCTCGTAT32526119.083036121308268TruSeq Adapter, Index 10 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG350950.068.5233848
GTATGCC351300.068.4829947
CGTATGC351550.068.4641546
ATGCCGT351900.068.3423949
TCGTATG352600.068.272245
CTGCTTG352350.068.1677159
CTCGTAT352350.068.0027844
TGCCGTC353550.067.9957450
TGCTTGA354100.067.8802560
GCCGTCT354700.067.7871451
GCAATCT350600.067.6653739
CCGTCTT355400.067.6634852
ATCTCGT354650.067.52228542
TCTCGTA355100.067.46628643
TCTGCTT356400.067.44218458
CAATCTC353650.067.2995340
GCTTGAA357450.067.2616961
AATCTCG354600.067.2475441
AGCAATC352800.067.21168538
CTTCTGC359700.066.7825656
Produced by FastQC (version 0.11.5)